Positive ANA workup – Is it lupus or not?

Read on to learn about newer tests that can help get a more accurate and faster diagnosis of lupus for a positive ANA workup

Microscopic view of a positive fluorescent antinuclear antibody test used to diagnose a lupus patient




The Positive ANA Workup Begins the Journey

If someone is found to have a positive antinuclear antibody (ANA), the doctor will often tell the patient they need a positive ANA workup and that “lupus needs to be ruled out.”
Lupus is a disease where the immune system of the affected person attacks their own body (called an autoimmune disease). If someone has symptoms suggestive of lupus (fatigue, joint pain, rash, fever, pleurisy, Raynaud’s, mouth sores, etc) the doctor will usually order an antinuclear antibody (ANA, also called FANA) test as depicted in the picture above. This is what the lab technician sees under the microscope when someone’s blood is positive for ANA. This is the initial test done in people who could possibly have systemic lupus erythematosus (SLE) because around 98% to 99% of adult SLE patients are positive for ANA (when both the IFA technique and ELISA lab methods are used). Children, especially young children, with SLE have higher rates of being ANA negative (as high as 15% to 20% in young children).


If positive, the patient is then often referred to a rheumatologist (the doctor who specializes in lupus)

Doctor with stethoscope around the neck and pens and a penlight in the white lab coat pocket




The Visit to the Rheumatologist: Where the positive ANA workup often begins

The rheumatologist then does a complete history (asks about symptoms), performs a thorough physical examination, and does a whole bunch of blood work and takes a urine sample to look for lupus. However, since multiple other disorders can cause similar symptoms as lupus and can also cause a positive ANA, he or she will also look for these possibilities. These tests include labs such as anti-dsDNA, anti-Smith antibody, anti-SSA, anti-RNP, rheumatoid factor, anti-CCP antibody, complete blood counts, liver enzymes, kidney function test, C3 and C4 complements, Coombs’ antibody, CH50 complement test, antiphospholipid antibodies, and a urine protein measurement. 


The list of disorders that can mimic lupus and can have a positive ANA is broad and include (not an exhaustive list):

– Other systemic autoimmune diseases (such as rheumatoid arthritis, scleroderma, Sjogren’s, polymyositis, mixed connective tissue disease, undifferentiated connective tissue disease)
– Infections (such as hepatitis C, hepatitis B, parvovirus infection, Epstein Barr virus infection)
– Fibromyalgia
– Cancer
– Medications

Or, the ANA can be a normal finding. It is found in up to 20% of all women. For example, a 55-year-old woman could have joint pains due to a different type of arthritis (such as osteoarthritis, OA), be very tired and fatigued after going through menopause, and have a positive ANA as a normal finding.

If the rheumatologist diagnoses lupus or a related disorder, hopefully, you will be treated immediately. Or, the rheumatologist will find another reason for your problems and the ANA (such as the woman with OA in the above paragraph).

A large black 6 painted on a white wall with a man in jeans, coat, and hat leans back on the wall with his head down




6 Years for a Diagnosis of Lupus?

Unfortunately, not everyone is so lucky. In 2014, UK Lupus did a survey of their members. Over 2500 SLE patients responded. The survey showed that it took an average of 6.4 years to get a correct diagnosis. Most ended up seeing multiple doctors before a diagnosis was reached. A few years later, the Lupus Foundation of America did a similar survey and found similar results, it took an average of 6 years. This is a travesty, and a huge unmet need. We need better ways to diagnose lupus. 


We do have better and more accurate ways, by the way, read onwards! But 1st … why does it take so long?

There are several reasons:

SLE will often start off slowly and add symptoms and problems and blood test results very slowly over time. Someone can actually have the disease lupus, feel miserable, yet not have enough evidence using the typical labs to make the diagnosis. The person often suffers while waiting for new problems to occur before enough evidence is present to properly make the diagnosis. The best solution for this problem is if we can figure out a way to identify the disease process of lupus earlier and faster by identifying an immune system abnormality that is unique for lupus. For example, maybe this could be a certain interferon signature in the blood work.  However, we don’t have this luxury yet. 

Another possible scenario is the person who has enough evidence for a systemic autoimmune disease, but there is not enough to label it as one of them (such as SLE, rheumatoid arthritis, scleroderma, Sjogren’s, or polymyositis). This person has evidence of an autoimmune disease, which we also call connective tissue diseases (CTDs), but it has not “differentiated” into one of the named CTDs. So, we label this person as having an undifferentiated connective tissue disease (UCTD). 

People in the above situations could heed my advice and see a rheumatologist who does certain procedures to try to get a faster diagnosis (discussed below).

Dr. Donald Thomas holding an ultrasound transducer on the foot of a patient along with the screen image of the sonogram




Musculoskeletal Ultrasound can look inside the Joints right in the Rheumatologist’s Exam Room

Many, but not most, rheumatologists can perform an ultrasound exam of the joints right in the examination room. In the photo above, I am doing an exam of the big toe joint in a patient. You can see a very nice picture of the joint ultrasound image on the screen. The vertical blue line in the middle cuts right between the two bones of the toe (that is where the cartilage of the joint exists). The horizontal bright white line going to the right is one toe bone (the middle phalanx) and the left line that starts out as a hump is the other (the first metatarsal bone). 


90% of adult SLE patients have inflammatory arthritis. They typically have pain in the joints and stiffness that is worse when they first wake up from sleep. The middle joints of the fingers, the knuckles, elbows, and knees are some of the most common joints affected. It can be very difficult to tell on physical examination (PE) when some SLE patients have inflammatory arthritis. They often will not have swelling of the joints on the PE. An ultrasound exam can be invaluable to diagnose arthritis accurately in these individuals. I’ve seen quite a few patients who were told they have fibromyalgia (which is not an arthritis) or arthralgias (joint pains without inflammatory arthritis) yet turn out to actually have lupus inflammatory arthritis easily seen on ultrasound.

Below is one of my SLE patient’s ultrasound results of the wrist (I have her permission to use her image). Note the horizontal white line in the center between the red and yellow arrows. Those are two bones of her wrist (bright white = bone). Just above it is an oval dark area (the red arrow points at it). That is swelling and fluid inside of her wrist joint (fluid shows up as black). This is diagnostic of arthritis. Yet, more telling is the orange blotches that are on the rim of the joint swelling (yellow arrow). That is actual lupus inflammation of the joint. This ultrasound result made it very easy to prove she has lupus inflammatory arthritis.

​(by the way, the upper orange blotch is blood in the vein just below the skin of her her hand. Look at the top of your hand. See those blue veins? Your veins would look like this under ultrasound). 

Ultrasound image of a wrist showing lupus arthritis (inflammatory synovitis) on Doppler




Finding a Rheumatologist Certified in Musculoskeletal Ultrasound (rhMSUS)

So, first, you can search out a rheumatologist who does ultrasound in his or her office. You can figure this out by going to the American College of Rheumatology’s website and look for a rheumatologist in your area who is certified to do these exams: https:///www.rheumatology.org/Learning-Center/RhMSUS-Certification/RhMSUS-Designees


You can then look up the names of doctors in your area to see if they are on the list. These rheumatologists can put the initials rhMSUS after the MD of their name (rhMSUS stands for Musculoskeletal Ultrasound Certification in Rheumatology). My partner (Dr. Yevgeniy Sheyn, MD) and I were in the very first group of doctors to earn this prestigious certification. 

The second best option is to call up rheumatology offices in your area and ask if their doctors do ultrasound exams. Most rheumatologists who perform ultrasound are not certified (and are not on this list), yet many of them are still excellent ultrasonographers. Ask the phone personnel who they would see in that office to get an US exam if they might have lupus. That is always a good question to ask. Office staff usually know who the best doctors to see in their practice are. 





Exagen Diagnostic’s AVISE Lupus Test and AVISE CTD Test for the “Positive ANA Workup”

The second test that can be very helpful is to get newer and more accurate labs done. A company called Exagen specializes in lupus lab tests. A type of blood test called Cell-Bound Complement Activation Products (CB-CAPs) is more sensitive and accurate than standard tests in diagnosing lupus compared to the usual commercial insurance labs (LabCorp  and Quest) that most doctors order from. Exagen put these valuable labs in two helpful tests called the AVISE Lupus Test and the AVISE CTD test. Anyone can get these tests done, yet only around 25% of rheumatologists order them.


Why do so few rheumatologists order these tests if they are so accurate in diagnosing SLE?

Previously, to order these tests, a rheumatologist had to have a lab technician in the office that could draw the blood, process it correctly, then send it to the Exagen labs in California. This is a lot of work. Most rheumatologists do not have the capability to do this, or do not want to put forth the extra effort and work to implement these tests in their office. (It can be a lot of hassle ordering new types of labs, fill out a lot of paperwork, and fight with the insurance companies to cover the lab costs for patients… so I can certainly understand why they would not want to do them!)

Below is an example of a patient who has a positive AVISE Lupus Test. The orange oval at the top left highlights this patient’s positive result and the middle circle highlights it on the graph. The bottom orange oval highlights her positive CB-CAPS (EC4d and BC4d) that gave her the positive results. The value of this test is that CB-CAPS become positive due to activation of and consumption of complements (important immune system proteins). This is a key problem in SLE (complement activation and lower C3 and C4). Most rheumatologists order C3 and C4 complements on standard tests hoping to see low C3 or C4 to help diagnose SLE. However, this only occurs in 20% to 30% of SLE patients because the liver will often times produce a whole bunch more C3 and C4, which ends up normalizing the blood levels. CB-CAPS (EC4d and BC4d) results are not interfered with by this liver over production of C3 and C4.

A positive AVISE Lupus Test result indicating a diagnosis of lupus




You can imagine how this test could simplify things. 

HOWEVER: a word of caution! … if you happen to get this test done, please do not assume that a positive AVISE Lupus Test automatically means you have lupus. On the other hand, a negative result does not mean you do not have it. The test result increases or decreases the likelihood of having lupus. For example, if the patient with test result above started off with a low likelihood of having SLE, this result may not be enough to say she has SLE. However, if the result was much further on the right of the graph (such as a Tier 1 positive test), then that could be enough to say she has SLE.  


So how can you get this valuable test done?

A middle aged white female doctor in a white lab coat talking to a black female lupus patient about a lupus diagnosis




How to get the AVISE Lupus Test or AVISE CTD Test

It is now much easier to get the AVISE Lupus Test done. If you are interested in doing this. Follow my advice. Doctors are much more likely to do something if you make it as easy for them to do as possible. All doctors are overworked these days. So, if you make it easy, then it is a cinch.


1. Download the order form from Exagen.: https://1rn4xv3o8v9yy7scj1xot3b1-wpengine.netdna-ssl.com/wp-content/uploads/LM1028_Universal_APN_0320-1.pdf

2. Fill out the form completely with all your information and the doctor’s information.

3. In Step 2 circle the diagnosis D89.89

4. Check the boxes that say “AVISE CTD Test” and the one underneath it that says “Add AVISE SLE Prognostic regardless of AVISE Index Result” and the “Add anti-Histone”

5. On page 3 print and sign your name. Fill out the last page if appropriate (sometimes you will get the test for free)

6. Make an appointment with your rheumatologist. Very tactfully ask, “Could I possibly get this lab test done for lupus called the AVISE test? I already filled out all the paperwork and I can get it done at a local lab”.  Then just have him or her write in their NPI number upper right side and sign anywhere on the first page.

Also (important) ask them to add the diagnostic code D89.89 to the clinic visit note for that day. 

7. Go to the lab locator online at: https://avisetest.com/telavise-draw-site-locator/

8. Type in your zip code to find the closest lab that can do the test. 

9. Call Exagen at 888-452-1522, option 3. Tell them you’d like to get the test done and you have the paperwork.  Ask them how much it will end up costing you with your insurance. Most people pay a copay of $0 to $45. If you have no insurance, I believe it is $100 as of 2020. Then ask them what your next steps are.

10. Go to the lab to get your lab drawn, then see your rheumatologist a couple of weeks later to discuss the results. 

Asian man giving the thumbs up sign





I strongly believe that people get better healthcare when they are proactive in their healthcare. If you have a positive ANA, have symptoms that could potentially be caused by lupus, had a thorough workup, yet you feel like you don’t have a good answer, take my advice.

– Find a rheumatologist who can do an ultrasound of your joints
– And get the AVISE CTD or AVISE Lupus test done

if you do this, then you can be confident that you have done everything that you can with today’s modern technology. I hope in the future that I can report on even better tests. A lot of research is being done looking into this, and I will certainly report on it when it becomes available.

I would love to hear from you about your experience. Write comments below under “LEAVE A REPLY”.  If you have lupus, how long did it take? Was it frustrating? What ended up being the key in getting your diagnosis correct? Also, if anyone has any questions, I’ll also be glad to answer them.

Please SHARE with anyone who is struggling to get a proper diagnosis after being ANA positive.

NOTE: I am on the Speaker’s Bureau for Exagen. I do believe strongly in how their tests improve patient care. Note, that the Lupus Foundation of America has also partnered with them to support their help for lupus patients. 

CB-CAPS became a reality partially due big efforts and funding from The Lupus Foundation of America.  Please join in you have lupus and are not a member. It is a wonderful organization fighting for your health!

Wooden Scrabble tiles spelling out SHARE





Donald Thomas, MD, author of “The Lupus Encyclopedia”

​Photo #1 credit (ANA picture): from Simon Caulton on Wikipedia.com

8 thoughts on “Positive ANA workup – Is it lupus or not?

  • September 21, 2020 at 10:01 pm

    Positive ANA result, referred by GP to a rheumatologist. Further labs showed B2GP and anticardiolipin antibodies, anti ds dna antibodies, pancytopenia, and low C3 and C4. First rheumatologist said “I’m not convinced it’s lupus, just keep an eye on all the labs.” Went to two more rheumatologists for opinions and just got notes saying “very perplexing 27-year old, currently at a loss as to the underlying etiology despite extensive workup for autoimmune, inflammatory, or connective tissue disease that might be associated with this manifestation.” Frustrated, flew across the country to a tertiary care center for a fourth opinion. The rheumatologist there said, “I don’t think you’re that perplexing, this is definitely rheumatic disease. You meet the criteria for SLE and likely will meet the scleroderma criteria as well within the next couple years. You need to be on hydroxychloroquine, and I’m very sorry no one has put you on it in the last two years.” I am persistent, highly educated, and have the resources to spend a lot of time and money getting a diagnosis. Yet it still took two years. Something needs to change.

    • September 22, 2020 at 9:25 am

      So sorry to hear that, ARW, that should never happen! I’m glad you finally found a good rheumatologist, but that should have occurred at the first visit from what you are describing. I hope you are doing better.

  • September 21, 2020 at 11:38 pm

    I’ve had Lupus for over 30 yrs and over the last several years have begun to really struggle with joint involvement in my hands. Rheumy thinks it’s more from Osteoarthritis. How do you determine if it’s coming from Osteo or your lupus?

  • September 22, 2020 at 9:31 am

    Sharri: Osteoarthritis is usually easy to diagnose on physical exam. We find bony swellings of the finger joints (the end joints and middle joints, but usually not the knuckles). For early OA, an ultrasound exam can be helpful if the bone spurs as too small to feel. X-rays can help in more advanced OA. The inflammatory arthritis of SLE is very different. It causes more prolonged stiffness in the AM and more severe than OA and on physical exam we find soft tissue rubbery feeling swelling of the middle joints, knuckles and wrists, but not the end joints … I hope that helps. btw… I am a strong believer in taking pharmaceutical grade glucosamine sulfate for OA as it may slow down the progressive loss of cartilage over time. Don’t take glucosamine hydrochloride (doesn’t work) nor poor quality brands (most are)… WynnPharma makes pharmaceutical grade products. I take their InvigoFlex AMPM: https:/www.wynnpharm.com/invigoflex-glucosamine-and-chondroitin-supplements/

  • September 23, 2020 at 9:23 am

    Over 30 years to get a diagnosis and this was from a General MD, formally an ER MD, turned Wholistic after a bout with cancer. Many years of flares, days off work, depression and pain lost jobs therefore loosing medical insurance. Not till Obama Care received insurance, part time work and out of the pocket to pay for this ER MD with a great reputation for actually helping people with unusual issues. He opted out of medical facilities to help his patience more. Fortunately I was able to push exercise, very healthy eating and pursued Traditional Chinese Medicine all these 30 plus years that helped me. Metabolically very healthy, many doctors still do not believe I have Lupus, RA, Sjogren’s, Raynard’s plus. Yet Avise, other blood tests and symptoms prove positive. All the many doctors I saw throughout the years surmised that I was depressed due to the depression and pain I had. Simple dismissal and no blood tests by doctors who regardless of being over worked, were negligent. I refuse to give up, I eat healthy, mainly an anti-inflammatory diet, exercise, and still pursue Chinese Medicine with hydroxychloroquine from France now. Do not trust doctors till proven ethical, conscientious and worthy of respect. Read your book twice, I continue to study everything I can regarding these conditions, including the immune system, gastro issues, hormones, and drugs. I am my best expert and trump any RA doctor with any amount of years of experience. I am the one who is intimate with what works and what does not and the idiosyncrasies of this complex disfunction.

    • September 23, 2020 at 6:10 pm

      Malka: Thanks for sharing your story, for persevering and being so proactive in your care. Hopefully, your drive will rub off on others, and I’m sure you do!

  • September 23, 2020 at 3:04 pm

    I was 16 at the first onset of symptoms. I was a gymnast. I had to quit due to no strength and pain in my hands. I started noticing I had no strength to hold the bars. Then the pain set in with no swelling in my hands. This was all attributed to training. Once I quit training, the swelling started in my ankles and knees.
    Then in my twenties, I started to experience pains in the sternum area. I had made 3 different trips to the ER over a span of my adult life thinking it was a heart attack. Everything came back ok and was told they were probably a panic attack. I have dealt most of my life with sternum pain. I also experienced dark bruises on my body.
    My turning point came when my husband passed when I was 52. I was thrown into the worst flare of my life without knowing it was a flare. I was told I was depressed and needed counseling to help deal with the grief. The sad part on this is my husband knew something was wrong with me. It was sad that he didn’t live long enough to find out what the diagnosis is. He saw me struggle with the pain, the swelling and dark bruising.
    When I couldn’t stand and my PCP wasn’t able to assist, I made an appointment with a rheumatologist on my own. It was a nine month wait to get in to see her.
    My first appointment consisted of her looking at each joint, touching, feeling the heat of my joints and asking so many questions my head was spinning. She concluded this appointment by telling me she believes we are dealing with one of 3 things…Gout, RA or Lupus. She set me up for labs and gave me a prescription for a high dose of NSAIDS then had me come back in a month to go over the findings.
    By my next appointment, I was feeling so good, I figured I was ok. She walked in the room and told me it was what she had expected, “You have Lupus.” I started crying. Not because I had Lupus. I really didn’t know what Lupus was. It was the fact that a doctor believed my pain was real. I was 52 years old. Thirty-six years I went from doctor to doctor with each one telling me to lose weight, go to counseling and then making me believe it was all in my head.
    I am a huge advocate now for Lupus. I don’t want anyone else to go thirty-six years in pain. I now have a fantastic PCP that does primary care for autoimmune patients. She is on the same portal as my rheumatologist. This in turn makes sure I have the best care possible. One thing my PCP does is have me to speak to medical students that come through her office about my Lupus. She is doing everything she can to inform new doctors how important it is to listen to a patient. I’m so very thankful for this.
    My vision is to see that every Lupus patient isn’t turned away without the help and resources he/she needs to walk the Lupus journey.

    • September 23, 2020 at 6:13 pm

      Thanks for sharing your story! I’m glad you finally found someone who believed you. I often hear the same from new patients. Lupus truly is an “invisible disease” even to some doctors and with many of the labs we use. Please keep up the advocacy, it is so important


Leave a Reply

Your email address will not be published. Required fields are marked *